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Expanding our understanding of marine viral diversity through metagenomic analyses of biofilms

  • Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42995-020-00078-4) contains supplementary material, which is available to authorized users.
  • Edited by Chengchao Chen.
  • Recent metagenomics surveys have provided insights into the marine virosphere. However, these surveys have focused solely on viruses in seawater, neglecting those associated with biofilms. By analyzing 1.75 terabases of biofilm metagenomic data, 3974 viral sequences were identified from eight locations around the world. Over 90% of these viral sequences were not found in previously reported datasets. Comparisons between biofilm and seawater metagenomes identified viruses that are endemic to the biofilm niche. Analysis of viral sequences integrated within biofilm-derived microbial genomes revealed potential functional genes for trimeric autotransporter adhesin and polysaccharide metabolism, which may contribute to biofilm formation by the bacterial hosts. However, more than 70% of the genes could not be annotated. These findings show marine biofilms to be a reservoir of novel viruses and have enhanced our understanding of natural virus-bacteria ecosystems.
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Expanding our understanding of marine viral diversity through metagenomic analyses of biofilms

    Corresponding author: Pei-Yuan Qian, boqianpy@ust.hk
    Corresponding author: Weipeng Zhang, zhangweipeng1987@126.com
  • 1. College of Marine Life Sciences, Ocean University of China, Qingdao 266100, China
  • 2. Institute for Advanced Ocean Study, Ocean University of China, Qingdao 266100, China
  • 3. Institute for Advanced Ocean Study, Ocean University of China, Qingdao 266100, China
  • 4. Fok Ying Tung Research Institute, Hong Kong University of Science and Technology, Guangzhou 510000, China
  • 5. Department of Mathematics, Hong Kong University of Science and Technology, Hong Kong, China
  • 6. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China

Abstract: Recent metagenomics surveys have provided insights into the marine virosphere. However, these surveys have focused solely on viruses in seawater, neglecting those associated with biofilms. By analyzing 1.75 terabases of biofilm metagenomic data, 3974 viral sequences were identified from eight locations around the world. Over 90% of these viral sequences were not found in previously reported datasets. Comparisons between biofilm and seawater metagenomes identified viruses that are endemic to the biofilm niche. Analysis of viral sequences integrated within biofilm-derived microbial genomes revealed potential functional genes for trimeric autotransporter adhesin and polysaccharide metabolism, which may contribute to biofilm formation by the bacterial hosts. However, more than 70% of the genes could not be annotated. These findings show marine biofilms to be a reservoir of novel viruses and have enhanced our understanding of natural virus-bacteria ecosystems.

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Introduction
  • Viruses make a significant contribution to nutrient and energy conversion processes in marine ecosystems via the modulation of the structure and functions of protistan, bacterial, and archaeal communities (Breitbart 2012; Kristensen et al. 2011; Suttle 2005; Zhang et al. 2014). The viral shunt releases around 10 billion tons of carbon per day and is probably a fundamental step in marine carbon cycling (Breitbart 2012). Viruses that infect bacterial hosts, known as phages, express auxiliary metabolic genes (AMGs) that influence the central metabolic processes of their hosts, such as photosynthesis and nutrient acquisition (Thompson et al. 2011; Xu et al. 2018).

    However, because of their large and highly dynamic populations, the vast majority of marine viruses remain unexplored. Great efforts have been spent on the isolation of viruses infecting many major marine bacterial lineages, such as Prochlorococcus (Sullivan et al. 2003), SAR11 (Zhao et al. 2013), and Roseobacter (Zhang et al. 2019a). Recent advances in culture-free approaches (e.g., metagenomics) have facilitated an unprecedented increase in the analysis of the diversity of marine microbes (bacteria and archaea) and viruses (reviewed in Coutinho et al. 2018). The global ocean dsDNA viromic dataset was established by the Tara Ocean Project with the goal of exploring ocean virus diversity to better understand the ecological and evolutionary drivers behind these viral communities and to reveal new mechanisms by which these viruses affect global oceanic microbial processes (Brum et al. 2015). In addition to the Tara Ocean Project, several other projects have revealed viruses to be the most abundant biological entities in marine ecosystems, e.g., in coral reefs (Thurber et al. 2017), and in marine sediments (Danovaro et al. 2008; Engelhardt et al. 2014).

    Most oceanic surveys have focused on the viruses infecting free-living bacterioplankton while those associated with biofilms were neglected. Biofilm formation confers several ecological advantages on bacteria and archaea, such as environmental protection, increased access to nutrients, and enhanced interspecies interactions (Dang and Lovell 2016). The individual and collective viral protection is conferred by the biofilm architecture (Vidakovic et al. 2018). Biofilms supported on artificial surfaces have been used as models to study biofilm developmental processes, microbe-invertebrate interactions, and novel microbial diversity and functions in marine environments (Chung et al. 2010; Salta et al. 2013; Zhang et al. 2015). In a recent study, Zhang et al. (2019b) examined 101 biofilm samples formed on man-made panels and natural rocks immersed in eight locations across the Atlantic, Indian, and Pacific Oceans and investigated the microbial (bacterial and archaeal) diversity and functional potential within these microbiomes. In the present study, we analyzed the viral sequences extracted from the same 101 biofilm metagenomes with the aim of obtaining a systematic understanding of viral diversity and function.

Results

    Metagenomic identification of viruses in marine biofilms

  • The locations of where the 101 biofilm samples were collected are shown (Fig. 1). The biofilms were developed on eight types of artificial substrates (polystyrene Petri dishes, zinc panels, aluminum, poly(ether-ether-ketone), polytetrafluoroethylene, poly(vinyl chloride), stainless steel, and titanium). The substrates were deployed at a depth of 1-2 m at eight locations around the world (the South Atlantic, the Red Sea, the waters off Hong Kong, Yung Shue O Bay, the East China Sea, and three sites in the South China Sea). Metagenome assembly generated 72, 132, 494 contigs in total, from which 3974 viral sequences (longer than 5.0 kbp) were predicted. The viral sequences had a maximum length of 351.55 kbp, an average length of 14.57 kbp, and an average cytosine bases (GC) content of 47.75%. The novelty of the viruses was evaluated by comparing the viral sequences with the Integrated Microbial Genome/Virus (IMG/VR) database, which contains sequences from almost 8500 isolated viruses and over 700, 000 viral contigs from metagenomes. Consistent with the rules used in a previous study (Paez-Espino et al. 2017), biofilm-derived viral sequences of over 1 kbp with 90% or higher similarity to sequences in the IMG/VR database were considered to be known viruses; only 358 (9.01%) biofilm-derived viral sequences were found in the IMG/VR database (Fig. 2).

    Figure 1.  Sampling locations of the 101 biofilms. The eight locations include (1) South Atlantic, (2) Red Sea, (3) Hong Kong Water, (4) Yung Shue O Bay, (5) East China Sea, (6) South China Sea 1, (7) South China Sea 2, and (8) South China Sea 3. Tara surface seawater samples used for comparison are also shown

    Figure 2.  Similarity between viral sequences identified from marine biofilms and those documented in the IMG/VR database. The biofilm-derived viral sequences were BLASTn searched against the IMG/VR database. The BLASTn hits with over 90% similarity for more than 1000 bp alignments were considered to be known viruses (dots in red), while the other hits (dots in blue) and those with no significant similarity were considered to be novel viruses

    74, 895 open reading frames (ORFs) were predicted from the biofilm-derived viral sequences. To confirm the VirSorter prediction, HMMER was used to search the ORFs against the virus orthologous groups (VOGs) database. As a result, all the viral sequences had ORFs that achieved hits in the VOG database. In total, 31, 038 VOG hits (41.44% of the total ORFs) were obtained, of which 2764 were non-redundant. The 30 most abundant VOGs consisted of genes encoding viral structural proteins, such as terminase large unit (VOG09355), base plate protein J (VOG00195), terminase large unit gp2 (VOG00080), and probable capsid protein gp17 (VOG02249) (Supplementary Fig. S1). The other abundant VOGs included genes responsible for DNA replication and transcription, such as DNA polymerase (VOG00073) (Supplementary Fig. S1).

    Taxonomic classification indicated that 81.60% of the VOGs were associated with Caudovirales, 0.32% were associated with Maveriviricetes, with the remaining VOGs considered to be unclassified viruses (Supplementary Fig. S2). Phylogenetic analysis using the terminase large subunit VOG9355, identified from the biofilm viral sequences and sequences from the VOG database, revealed three relatively independent branches formed by the biofilm viruses, most likely representing novel viral lineages (Fig. 3).

    Figure 3.  Phylogenetic tree of the terminase large subunit gene VOG9355 identified from the biofilm phage sequences. Closely related terminase large subunit gene sequences documented in the VOG database were revealed by hmmscan and then used as a reference. The protein sequences that could be aligned by ClustalW were used to construct a maximum likelihood tree with 1000 replicates. Bootstrap values (> 50) are shown on the branches. All the gene sequences from biofilms are shown in blue, and branches that represent potentially novel viral lineages are shown in red

  • Endemism of the viruses to marine biofilms

  • To explore the niche specificity of the viruses detected in the biofilms, the abundance of the biofilm-derived viruses was investigated by mapping the metagenomic reads of the 101 biofilm and 91 seawater samples (10 million reads per sample) to the viral sequences. To this end, 250 viral sequences with coverage > 1 in at least one biofilm and coverage = 0 in all seawater samples were identified (Fig. 4), suggesting the existence of viruses that are endemic to the biofilm niche. To confirm this result, five phages that were abundant in the Red Sea biofilms were selected their distribution in nine Red Sea biofilm samples and nine adjacent seawater samples were investigated. The number of reads mapped to these phages exceeded 100 in almost all of the biofilm samples but was close to zero in the seawater metagenomes (Supplementary Fig. S3).

    Figure 4.  The endemism of biofilm-derived viruses. Metagenomic reads of 101 biofilm and 91 seawater samples were mapped to the biofilm-derived viral contigs to compare their abundance in biofilms and seawater. Metagenomic reads (101 bp and ten million reads per sample) were mapped to gene sequences. Viral sequences with coverage > 1 in biofilms and coverage = 0 in seawater samples are presented

  • Viruses in single genomes and their functions

  • To investigate the hosts of the viruses and the potential virus-host interactions, 479 microbial genome bins extracted from the biofilm metagenomes were analysed. These genome bins belonged to 20 different microbial phyla, including Proteobacteria (272 genomes), six 'Candidatus' phyla (7 genomes), Acidobacteria (6 genomes), Actinobacteria (10 genomes), Bacteroidetes (100 genomes), Cyanobacteria (34 genomes), Deinococcus-Thermus (1 genome), Firmicutes (2 genomes), Lentisphaerae (2 genomes), Parcubacteria (1 genome), Planctomycetes (22 genomes), Rhodothermaeota (1 genome), Verrucomicrobia (19 genomes), and Euryarchaeota (2 genomes) (Supplementary Fig. S4). Viral sequences from the genome bins were identified using the software PHASEER (McCoy et al. 2007), which was designed for mining phage sequences from draft genomes. In total, 149 phage sequences were distributed in 101 bacterial genome bins of Alphaproteobacteria, Gammaproteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Candidatus, Gracilibacteria, Cyanobacteria, Firmicutes, Lentisphaerae, Oligoflexia, Planctomycetes, and Rhodothermaeota (Fig. 5a). Within these taxa, Gammaproteobacteria (n = 43), followed by Alphaproteobacteria (n = 30), possessed the largest number of phage-containing genome bins (Fig. 5a). The GC content of the phage contigs was compared with that of the bacterial genomes and found to be very similar (Supplementary Fig. S5).

    Figure 5.  Identification of viral sequences from microbial genome bins and viral gene functional annotation. A Viral sequences were identified from 100 microbial genomes distributed across 11 bacterial phyla (Proteobacteria were divided into Alpha- and Gamma-proteobacteria). B The number of viral genes annotated by BLASTp searching against the COG database for functional classification

    To detect potential functions encoded by these phages, all genes derived from the phage sequences (4121 predicted ORFs) were analyzed by classifying the gene functions using the COG database (Galperin et al. 2015; Tatusov et al. 2000), which resulted in 22 COG categories (Fig. 5b). In total, 1023 ORFs (24.82%) resulted in hits in the COG database; however, 521 ORFs were classified as "general function" predictions only [R] or as "function unknown" [S]. Of the remaining 502 COGs, 40 were classified as being involved in amino acid transport and metabolism [E], nucleotide transport and metabolism [F], or as carbohydrate transport and metabolism [G], such as the genes encoding Na+/glutamate symporter [COG0786], deoxynucleotide kinases [COG1428], and chitinase [COG3325] (Fig. 5b).

    The functions of all genes derived from these phage contigs (4121 predicted ORFs) were further analyzed by searching them against the KEGG (Kanehisa et al. 2017) and CAZy databases (Lombard et al. 2014). The genes were characterized by searching the 1062 ORFs against the KEGG database's annotated sequences and the top 18 abundant KEGG matches are shown in Fig. 6. Interestingly, the most abundant KEGG hit was for trimeric autotransporter adhesin (K21449) and the genes for viral structure (e.g., K06909), transcriptional regulation (e.g., ParB family transcriptional regulator, chromosome partitioning protein K03497) and DNA replication (e.g., putative DNA primase/helicase K06919) were also annotated. KEGG annotation also revealed uncharacterized but relatively conserved genes (n = 96; 9.0% of all KEGG hits), such as K06903, K06907, and K06904. In parallel, 351 ORFs were annotated by CAZy: these mostly included genes for lysozymes, chitinases, lyase, and peptidoglycan lytic transglycosylases (Supplementary Fig. S6). In total 1133 ORFs were annotated by the CAZy or KEGG database, while the remaining 72.51% achieved no hits.

    Figure 6.  Potential auxiliary metabolic genes of phage genes extracted from the bacterial bins. The gene functions were predicted by BLASTp searching against the KEGG database. The top 18 (gene number > 10) KEGGs are shown

Discussion
  • The finding here that biofilms are composed of a number of previously unknown viruses is consistent with the notion that biofilm formation promotes virus accumulation and may be a potential library of infectious pathogens (Bettarel et al. 2006). When the biofilm-derived viral sequences were aligned with the VOG database, the most abundant genes were found to be related to structure and replication. More specifically, the base plate is a part of tailed prokaryotic viruses, such as Caudovirales, and it suggests the prevalence of tailed viruses in marine biofilms. The terminase large subunit is a viral DNA-packaging motor, which cleaves viral DNA into smaller pieces and inserts them into a procapsid powered by ATP hydrolysis (Rao and Feiss 2008). Capsid proteins encoded by relatively short genes function to protect nucleic acids and the tertiary structure of capsid proteins contain all the information required for virus assembly (Hagan and Zandi 2016). The annotation of these VOGs validates the conserved structure and function of biofilm-derived viruses; however, phylogenetic analysis of these proteins also indicates the existence of novel viral lineages in marine biofilms.

    There have been few studies reporting on virus endemism in environmental niches. In this study, it is shown that biofilm virus endemism is much greater than in seawater: 250 viral sequences were present in the biofilms collected from the different oceans, but they were absent from all the seawater samples. While surface-associated microbes and viruses must be seeded from seawater, many viruses are very scarce in seawater and so are unlikely to be sampled. Extracellular DNA released through cell lysis mediated by phages has been shown to enhance biofilm formation (Gödeke et al. 2011). Certain viruses are capable of forming biofilm-like assemblies for propagation (Thoulouze and Alcover 2011). In addition, phages can select for a mucoid bacterial phenotype to co-evolve and induce biofilm formation (Scanlan and Buckling 2012). One of the underlying mechanisms coordinating this relationship between viruses and biofilms involves quorum-sensing signals, which upregulate the expression of CRISPR-related genes (Høyland-Kroghsbo et al. 2017; Patterson et al. 2016) and decrease the level of phage receptors (Høyland-Kroghsbo et al. 2013; Tan et al. 2015). Another reason why so many novel viruses were discovered in biofilms is the seawater filtering process, which can highly concentrate the low abundant viruses that are missed during seawater sampling.

    According to previous metagenomic analyses of marine viruses (Coutinho et al. 2017; Mizuno et al. 2013), Cyanobacteria, Actinobacteria, Alphaproteobacteria, Gammaproteobacteria, and Verrucomicrobia are the most prevalent phage hosts. Results presented here are consistent with previous reports with Alpha- and Gamma-proteobacteria being the major hosts of phages in the biofilms. The proportion of guanine and GC content in DNA provides survival advantages in the adaption to environmental conditions (Almpanis et al. 2018; Mann and Chen 2010). Results presented here show a similar GC content between the phages and their hosts, suggesting that the viruses have adapted to their hosts and that certain environmental factors have had roles in shaping the intimate relationships between the phages and the bacteria in the biofilms (Motlagh et al. 2017). Viral sequences identified from microbial genomes are probably phages; however, due to technical limitations, it is difficult to extract all the genomes from metagenomes and distinguish all the phages from free viruses.

    With regard to phage function, more than 70% of the ORFs could not be annotated by the COG, KEGG, or CAZy databases, indicating the limited understanding of the function of biofilm-derived viruses and the need for additional experimental research. COG annotation suggested that the phages inhabiting biofilms may encode enzymes involved in central carbon metabolism. No phage genes for photosynthesis were detected, suggesting that the phages contribute little to carbon fixation in the biofilm communities, which is in contrast to previous findings that showed photosynthetic genes are prevalent in phages infecting subtidal microbial communities (McMinn et al. 2020; Sullivan et al. 2005; Thompson et al. 2011). Notably, 89 genes were found to code for trimeric autotransporter adhesin (K21449), which is a trimeric autotransporter that promotes biofilm formation in bacteria (Fey et al. 2002; Luqman et al. 2018; Raghunathan et al. 2011); mutation of this gene abolished the ability of biofilms to attach to plastic surfaces (Lazar Adler et al. 2013); over-expression of this gene in Salmonella enterica increased cell aggregation and adhesion to human intestinal Caco-2 epithelial cells (Raghunathan et al. 2011). Similarly, a recent study showed that SadA-expressing Staphylococci from the human gut showed increased cell adherence and internalization (Luqman et al. 2018). The high abundance of K21449 indicates the role of phages in facilitating biofilm formation by the bacterial hosts and thus provides clues to the specificity of the viral sphere in marine biofilms. Transcriptional regulators may also have significant mediating effects on the interactions between human beings and Epstein-Barr viruses (Arvey et al. 2012); however, the function of transcriptional regulators in marine viruses is unclear. Furthermore, the polysaccharide metabolism genes (e.g., chitinases) annotated by CAZy are probably used by phages to lyse hosts and are involved in carbon recycling within the biofilm communities.

Conclusions
  • Here we found that over 90% of the biofilm-derived viruses had no overlap with the IMG/VR database and provided evidence for the existence of viruses endemic to biofilms, suggesting that biofilm formation enables the discovery and reconstruction of viral genomes from marine environments. We identified potential auxiliary metabolic genes for trimeric autotransporter adhesin and polysaccharide metabolism in viral sequences integrated into the biofilm-derived microbial genomes, suggesting that phages may contribute to biofilm formation by the bacterial hosts, yet more than 70% of the phage genes functions remain unknown. Taken together, the present study has unveiled a hidden marine virosphere with novel viral diversity and unexplored functions.

Materials and methods

    Sampling

  • The biofilms were developed on eight types of artificial substrates: polystyrene petri dishes (9 × 1.2 cm), zinc panels (11 × 11 cm), aluminum, poly(ether-ether-ketone), polytetrafluoroethylene, poly(vinyl chloride), stainless steel, and titanium (5 × 5 cm). The artificial substrates were deployed at a depth of 1-2 m at eight locations around the world: the South Atlantic, the Red Sea, the waters off Hong Kong, Yung Shue O Bay, the East China Sea, and three sites in the South China Sea. The petri dishes were immersed in seawater for 12 days to allow for biofilm formation; the other artificial substrates were immersed for 30 days to allow for visible bacterial attachment. Biofilms that had formed on natural rocks were also collected. After collection, the biofilms were immediately transferred to the laboratory, and the surface bacterial cells were removed using sterile cotton tips and stored in 5 ml of DNA storage buffer (500 mmol/L NaCl, 50 mmol/L Tris-HCl, 40 mmol/L EDTA, and 50 mmol/L glucose) at − 80 ℃. During biofilm development, adjacent seawater samples were collected and successively filtered through 0.1-μm polycarbonate membrane filters (Millipore, Massachusetts, USA). The filters were stored in 5 ml of DNA storage buffer at − 80 ℃. In total, 101 biofilms and 24 seawater samples were collected. Additionally, 67 Tara seawater samples collected from marine surface (Sunagawa et al. 2015) were also used for comparisons between the biofilms and seawater (Supplementary Table S1).

  • DNA extraction and sequencing

  • Biofilms from the cotton tips and seawater samples on the filters were re-suspended in Tris-HCl buffer, pelleted by centrifugation at 4000 g for 10 min and then lysed with lysozyme (37 ℃ for 30 min) and the lysis buffer provided by the TIANamp Genomic DNA Kit (Tiangen Biotech, Beijing, China). Then, DNA extraction was performed using the TIANamp Genomic DNA Kit, following the manufacturer's protocol. DNA sequencing for the Red Sea samples was performed at the Beijing Genomics Institute (BGI, Beijing, China), and the other samples were sequenced at the Novogene Bioinformatics Institute (Novogene, Beijing, China). After the construction of 350-bp insert libraries, the DNA was sequenced on the HiSeq X Ten System at Novogene and the HiSeq 2500 System at BGI. Quality control was performed on a local server using the software NGS QC Toolkit (version 2.0) (Patel and Jain 2012) to remove low-quality reads (assigned by a quality score < 20 for > 30% of the read length) or unpaired high-quality reads. Information on metagenomic reads is given in Supplementary Table S1.

  • Metagenomic assembly and microbial genome binning

  • Following quality control, reads from the biofilm metagenomes were assembled into contigs using the software MEGAHIT (version 1.0.2) (Li et al. 2015) with kmer values of 21-121, increasing in steps of 10. Coverage information was generated by mapping metagenomic reads to the contigs using Bowtie2 (fastq as input format under a sensitive-local model). The contigs as well as the coverage information were used as input for MaxBin (version 2.0) (Wu et al. 2016) to assign the contigs to single genomes. The single genomes were further analyzed using MetaBAT for purification. The completeness and contamination of the genome bins were analyzed using CheckM (Parks et al. 2015). Duplicated genomes were removed based on the average nucleotide identity (ANI) information provided by the ANI calculator (Yoon et al. 2017), where genome pairs with ANI values exceeding 0.99 were taken as redundant genomes. Information of the assembled metagenomic contigs is given in Supplementary Table S2. Information on the genome bins is provided in Supplementary Table S3.

  • Viral sequences prediction and annotation

  • The software VirSorter (version 1.0.5) (Roux et al. 2015), installed on a local server, was used to identify viral sequences from the metagenomic contigs and genome bins. The database 'Refseqdb' and the mode 'BLASTp' were used for mining viral sequences, and only viruses in the categories of 'sure' or 'somewhat sure' were retained for the following analyses. Metagenomic reads of 101 biofilms and 91 seawater samples were mapped to the viral sequences using bbmap (version 2) (Bushnell 2014) to indicate viral coverage in biofilms and seawater (minimum alignment identity = 0.76). All the metagenomes for mapping were normalized to 10 million reads per metagenome, and all reads were trimmed to 101 bp in length by NGS QC Toolkit (version 2.0). The viral ORFs were predicted using Prodigal (version 2.0) (Hyatt et al. 2010) in the Meta model (only closed ends were allowed). A HMMER hmmscan (Johnson et al. 2010) against the VOG database (https://vogdb.org) was performed to classify the ORFs using an e-value cutoff of 1e − 7, and then the taxonomic affiliation was examined by MEGAN (Huson et al. 2016). The reference genes were selected from VOG database with hmmscan, and a phylogenetic tree was established with ClustW and 1000 bootstraps by MEGA 6 (Tamura et al. 2013). For potential function mining, annotation of the phage genes was performed by BLASTp (e value 1e − 7) searching against the COG (Galperin et al. 2015; Tatusov et al. 2000), KEGG (Kanehisa et al. 2017), and CAZy (Lombard et al. 2014) databases. The workflow of the present study is summarized in Supplementary Fig. S7.

Acknowledgements
  • The authors are grateful to a grant from the National Key Research and Development Program of China (2018YFC0310600) and two grants from Ocean University of China (841912035 and 842041010) to W.Z. The authors are also grateful to a grant from China Ocean Mineral Resources Research and Development Association (DY135-B2-03) and a grant from the Hong Kong Branch of South Marine Science and Engineering Guangdong Laboratory (SMSEGL20SC01) to P.Y.Q.

Author contributions
  • WZ, P-YQ, and ZR designed the project; WD, RW, and ZL performed the analysis; WD, RW, and WZ wrote the manuscript.

Data availability
Compliance with ethical standards

    Conflict of interest

  • The authors declare that they have no conflict of interest.

  • Animal and human rights statement

  • This article does not contain any studies with human participants or animals performed by any of the authors.

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