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Metabolic diversiication of anaerobic methanotrophic archaea in a deep-sea cold seep

  • Corresponding author: Yong Wang, wangy@idsse.ac.cn
  • Received Date: 2020-04-07
    Fund Project:

    We are grateful to Dr. J. W. Qiu for the sample collection during a Jiaolong manned submersible dive. This study was supported by the National Key Research and Development Program of China (2018YFC0310005 and 2016YFC0302500), and the National Science Foundation of China (No. 31460001 and No. 41476104).

  • Anaerobic methanotrophic archaea (ANME) can assimilate methane and govern the greenhouse efect of deep-sea cold seeps. In this study, a total of 13 ANME draft genomes representing ive ANME types (ANME-1a, ANME-1b, ANME-2a, ANME-2b and ANME-2c), in size between 0.8 and 1.8 Mbp, were obtained from the Jiaolong cold seep in the South China Sea. The small metagenome-assembled genomes (MAGs) contained all the essential pathways for methane oxidization and carbon dioxide ixation. All genes related to nitrate and sulfate reduction were absent from the MAGs, indicating their syntrophic dependence on partner organisms. Aside from acetate secretion and sugar storage, propanoate synthesis pathway, as an alternative novel carbon low, was identiied in all the MAGs and transcriptionally active. Regarding type-speciic features of the MAGs, the genes encoding archaellum and bacteria-derived chemotaxis were speciic to ANME-2, perhaps for itness under luctuation of methane and sulfate concentration lux. Our genomic and transcriptomic results strongly suggested that ANME could carry out simple carbon metabolism from C1 assimilation to C3 biosynthesis in the SCS cold seep, which casts light on a novel approach for synthetic biology.
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Metabolic diversiication of anaerobic methanotrophic archaea in a deep-sea cold seep

    Corresponding author: Yong Wang, wangy@idsse.ac.cn
  • 1 Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, Hainan, China;
  • 2 HKUST-CAS Sanya Joint Laboratory of Marine Science Research, Chinese Academy of Sciences, Sanya 572000, Hainan, China
Fund Project:  We are grateful to Dr. J. W. Qiu for the sample collection during a Jiaolong manned submersible dive. This study was supported by the National Key Research and Development Program of China (2018YFC0310005 and 2016YFC0302500), and the National Science Foundation of China (No. 31460001 and No. 41476104).

Abstract: Anaerobic methanotrophic archaea (ANME) can assimilate methane and govern the greenhouse efect of deep-sea cold seeps. In this study, a total of 13 ANME draft genomes representing ive ANME types (ANME-1a, ANME-1b, ANME-2a, ANME-2b and ANME-2c), in size between 0.8 and 1.8 Mbp, were obtained from the Jiaolong cold seep in the South China Sea. The small metagenome-assembled genomes (MAGs) contained all the essential pathways for methane oxidization and carbon dioxide ixation. All genes related to nitrate and sulfate reduction were absent from the MAGs, indicating their syntrophic dependence on partner organisms. Aside from acetate secretion and sugar storage, propanoate synthesis pathway, as an alternative novel carbon low, was identiied in all the MAGs and transcriptionally active. Regarding type-speciic features of the MAGs, the genes encoding archaellum and bacteria-derived chemotaxis were speciic to ANME-2, perhaps for itness under luctuation of methane and sulfate concentration lux. Our genomic and transcriptomic results strongly suggested that ANME could carry out simple carbon metabolism from C1 assimilation to C3 biosynthesis in the SCS cold seep, which casts light on a novel approach for synthetic biology.

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