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Eco-friendly preparation of chitooligosaccharides with different degrees of deacetylation from shrimp shell waste and their effects on the germination of wheat seeds
Xiaodan Fu, Lin Zhu, Li Li, Tan Zhang, Meng Li, Haijin Mou
2019, 1(1): 95-103.  doi: 10.1007/s42995-019-00012-3
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Recent advances in amino acid sensing and new challenges for protein nutrition in aquaculture
Chengdong Liu, Xuan Wang, Huihui Zhou, Kangsen Mai, Gen He
2019, 1(1): 50-59.  doi: 10.1007/s42995-019-00022-1
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Morphogenesis: a focus on marine invertebrates
Zhiyi Lv, Qiongxuan Lu, Bo Dong
2019, 1(1): 28-40.  doi: 10.1007/s42995-019-00016-z
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Cost-reduction strategies in massive genomics experiments
Haichao Li, Kun Wu, Chenchen Ruan, Jiao Pan, Yujin Wang, Hongan Long
2019, 1(1): 15-21.  doi: 10.1007/s42995-019-00013-2
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Our recent progress in epigenetic research using the model ciliate, Tetrahymena thermophila
Ting Cheng, Yuanyuan Wang, Jie Huang, Xiao Chen, Xiaolu Zhao, Shan Gao, Weibo Song
2019, 1(1): 4-14.  doi: 10.1007/s42995-019-00015-0
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Microbial assembly, interaction, functioning, activity and diversifcation: a review derived from community compositional data
Jiwen Liu, Zhe Meng, Xiaoyue Liu, Xiao-Hua Zhang
2019, 1(1): 112-128.  doi: 10.1007/s42995-019-00004-3
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Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafsh
Lei Wang, Jae Young Oh, Hye-Won Yang, Hyun Soo Kim, You-Jin Jeon
2019, 1(1): 104-111.  doi: 10.1007/s42995-019-00006-1
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Metabolites from marine invertebrates and their symbiotic microorganisms: molecular diversity discovery, mining, and application
Lu Liu, Yao-Yao Zheng, Chang-Lun Shao, Chang-Yun Wang
2019, 1(1): 60-94.  doi: 10.1007/s42995-019-00021-2
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Expanding our understanding of marine viral diversity through metagenomic analyses of biofilms
Wei Ding, Ruojun Wang, Zhicong Liang, Rui Zhang, Pei-Yuan Qian, Weipeng Zhang
Corrected proof  doi: 10.1007/s42995-020-00078-4
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Abstract:
Recent metagenomics surveys have provided insights into the marine virosphere. However, these surveys have focused solely on viruses in seawater, neglecting those associated with biofilms. By analyzing 1.75 terabases of biofilm metagenomic data, 3974 viral sequences were identified from eight locations around the world. Over 90% of these viral sequences were not found in previously reported datasets. Comparisons between biofilm and seawater metagenomes identified viruses that are endemic to the biofilm niche. Analysis of viral sequences integrated within biofilm-derived microbial genomes revealed potential functional genes for trimeric autotransporter adhesin and polysaccharide metabolism, which may contribute to biofilm formation by the bacterial hosts. However, more than 70% of the genes could not be annotated. These findings show marine biofilms to be a reservoir of novel viruses and have enhanced our understanding of natural virus-bacteria ecosystems.
High chromosomal evolutionary dynamics in sleeper gobies (Eleotridae) and notes on disruptive biological factors in Gobiiformes karyotypes (Osteichthyes, Teleostei)
Simião Alefe Soares da Silva, Paulo Augusto de Lima-Filho, Clóvis Coutinho da Motta-Neto, Gideão Wagner Werneck Félix da Costa, Marcelo de Bello Cioffi, Luiz Antônio Carlos Bertollo, Wagner Franco Molina
Corrected proof  doi: 10.1007/s42995-020-00084-6
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Abstract:
The order Gobiiformes is made up of more than 2200 species, representing one of the most diverse groups among teleost fishes. The biological causes for the tachytelic karyotype evolution of the gobies have not yet been fully studied. Here we expanded cytogenetic data for the Eleotridae family, analyzing the neotropical species Dormitator maculatus, Eleotris pisonis, Erotelis smaragdus, and Guavina guavina. In addition, a meta-analytical approach was followed for elucidating the karyotype diversification versus biological aspects (habitat and egg type) of the Gobiiformes. The species E. smaragdus and E. pisonis present 2n = 46 acrocentric chromosomes (NF = 46), D. maculatus 2n = 46 (36sm + 4st + 6a; NF = 86), and G. guavina, the most divergent karyotype, with 2n = 52 acrocentric chromosomes (NF = 52). Besides numeric and structural diversification in the karyotypes, the mapping of rDNAs and microsatellites also showed noticeable numerical and positional variation, supporting the high chromosomal evolutionary dynamism of these species. In Gobiiformes, karyotype patterns which are more divergent from the basal karyotype (2n = 46a) are associated with characteristics less effective to dispersion, such as the benthic habit. These adaptive characteristics, connected with the organization of the repetitive DNA content in the chromosomes, likely play a synergistic role in the remarkable karyotype diversification of this group.
The parasitic dinoflagellate Hematodinium infects marine crustaceans
Caiwen Li, Meng Li, Qian Huang
Corrected proof  doi: 10.1007/s42995-020-00061-z
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Abstract:
Hematodinium is a type of parasitic dinoflagellate that infects marine crustaceans globally. The parasite lives mainly in the hemolymph or hemocoels of affected hosts, and results in mortalities due to malfunction or loss of functions of major organs. In recent years, the parasite had developed into an emerging epidemic pathogen not only affecting wild populations of economically valuable marine crustaceans in western countries but also the sustainable yield of aquaculture of major crabs in China. The epidemics of the parasitic diseases expanded recently in the coastal waters of China, and caused frequent outbreaks in aquaculture of major crab species, especially Portunus trituberculatus and Scylla paramamosain. In addition, the pathogen infected two species of co-cultured shrimps and multiple cohabitating wild crabs, implying it is a significant threat to the sustainable culture of commercially valuable marine crustaceans. In particular, the polyculture system that is widely used along the coast of China may facilitate the spread and transmission of the pathogen. Thus, to provide a better understanding of the biological and ecological characteristics of the parasitic dinoflagellate and highlight important directions for future research, we have reviewed the current knowledge on the taxonomy, life cycle, pathogenesis, transmission and epidemiology of Hematodinium spp. Moreover, ecological countermeasures have been proposed for the prevention and control of the emerging infectious disease.
A new hypotrich ciliate, Oxytricha xianica sp.nov., with notes on the morphology and phylogeny of a Chinese population of Oxytricha auripunctata Blatterer & Foissner, 1988(Ciliophora, Oxytrichidae)
Jingyi Wang, Tengteng Zhang, Fengchao Li, Alan Warren, Yanbo Li, Chen Shao
Corrected proof  doi: 10.1007/s42995-020-00089-1
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Abstract:
The morphology and infraciliature of the new hypotrichous ciliate, Oxytricha xianica sp.nov., and a Chinese population of Oxytricha auripunctata Blatterer & Foissner, 1988, are investigated using live observation and protargol staining.Oxytricha xianica sp.nov.is defined by the body size 85-130 μm × 30-50 μm in vivo, the typical oxytrichid 18 frontoventral-transverse cirri, cortical granules absent, undulating membranes reduced or absent, three caudal cirri, dorsal kineties in Oxytricha pattern, and two ellipsoidal macronuclear nodules.The Chinese population of O.auripunctata differs slightly from other populations in the arrangement and color of cortical granules and position of anterior end of dorsal kinety 4.Phylogenetic analyses based on SSU-rDNA sequences support the assertion that O.auripunctata belongs to Oxytricha and Oxytrichidae.
Reproductive biology of female sawcheek scorpionfish, Brachypterois serrulata (Richardson, 1846) (Teleostei; Scorpaenidae) from Visakhapatnam Coast, India
Muddula Krishna Naranji, Govinda Rao Velamala, Kandula Sujatha
Corrected proof  doi: 10.1007/s42995-020-00083-7
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Abstract:
We examined the reproductive biology of sawcheek scorpionfish, Brachypterois serrulata, collected from Visakhapatnam fish landing center (fishing area covered 16.98°-20.20°N, 82.19°-86.53°E). We studied the annual reproductive cycle, fecundity, size at first maturity, mode of spawning with periodical changes and histological preparations. Size distribution and size at sexual maturity were calculated as 108 mm TL for females. The mean gonado somatic index values of female increased rapidly from January to December, and reached its peak during January and February. The fecundity of the fish species is determined by the size and weight of the individuals. The fecundity varied from 1896 to 20, 488, with an average of 5721.90 ova.
Growth, serum biochemical parameters, salinity tolerance and antioxidant enzyme activity of rainbow trout (Oncorhynchus mykiss) in response to dietary taurine levels
Ming Huang, Xiaogang Yang, Yangen Zhou, Jian Ge, D. Allen Davis, Yunwei Dong, Qinfeng Gao, Shuanglin Dong
Corrected proof  doi: 10.1007/s42995-020-00088-2
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Abstract:
This study evaluated the effect of dietary taurine levels on growth, serum biochemical parameters, salinity adaptability, and antioxidant activity of rainbow trout (Oncorhynchus mykiss). Four diets were formulated with taurine supplements at 0, 0.5, 1, and 2% w/v (abbreviated as T0, T0.5, T1, and T2, respectively). Rainbow trouts (initial weight of 80.09 ± 4.72 g) were stocked in tanks (180 L capacity), and were fed these diets for six weeks and subsequently underwent salinity acclimation. Physiological indicators were determined before salinity acclimation at 1, 4, 7, and 14 days afterwards. Results showed that there were no significant differences in growth performance (final mean weight ranged from 182.35 g to 198.48 g; percent weight gain was between 127.68% and 147.92%) of rainbow trout in freshwater stage, but dietary taurine supplement significantly increased serum-free taurine content. After entering seawater, the Na+-K+-ATPase activity of T2 group returned to its freshwater levels, and the serum cortisol content was significantly higher than T0 and T0.5 groups. At the end of this experiment, the liver superoxide dismutase activity in the T0 and T0.5 groups was significantly lower than in the T1 and T2 groups, and the liver catalase in the T0 group was the lowest whereas that in the T2 group was the highest. Muscle malondialdehyde content was the highest in the T0 group, and the lowest in the T2 group. Based on the results of this study, supplement of dietary taurine (0.5-2%) enhanced the salinity tolerance in rainbow trout, which increased with the higher taurine concentration.
Cultivation of uncultured marine microorganisms
Fengping Wang, Meng Li, Li Huang, Xiao-Hua Zhang
2021, 3(2): 117-120.   doi: 10.1007/s42995-021-00093-z
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Strategies for culturing active/dormant marine microbes
Da-Shuai Mu, Yang Ouyang, Guan-Jun Chen, Zong-Jun Du
2021, 3(2): 121-131.   doi: 10.1007/s42995-020-00053-z
Abstract(369) HTML PDF Springerlink
Abstract:
Microorganisms are ubiquitous in the ocean environment and they play key roles in marine ecosystem function and service. However,many of their functions and phenotypes remain unknown because indigenous marine bacteria are mostly difficult to culture. Although many novel techniques have brought previously uncultured microbes into laboratory culture,there are still many most-wanted or key players that need to be cultured from marine environments. This review discusses possible reasons for 'unculturable microbes' and categorizes uncultured bacteria into three groups: dominant active bacteria,rare active bacteria,and dormant bacteria. This review also summarizes advances in cultivation techniques for culturing each group of unculturable bacteria. Simulating the natural environment is an effective strategy for isolating dominant active bacteria,whereas culturomics and enrichment culture methods are proposed for isolating rare active bacteria. For dormant bacteria,resuscitation culture is an appropriate strategy. Furthermore,the review provides a list of the most-wanted bacteria and proposes potential strategies for culturing these bacteria in marine environments. The review provides new insight into the development of strategies for the cultivation of specific groups of uncultured bacteria and therefore paves the way for the detection of novel microbes and their functions in marine ecosystems.
From ecophysiology to cultivation methodology: filling the knowledge gap between uncultured and cultured microbes
Nimaichand Salam, Wen-Dong Xian, Mipeshwaree Devi Asem, Min Xiao, Wen-Jun Li
2021, 3(2): 132-147.   doi: 10.1007/s42995-020-00064-w
Abstract(2330) HTML PDF Springerlink
Abstract:
Earth is dominated by a myriad of microbial communities, but the majority fails to grow under in situ laboratory conditions. The basic cause of unculturability is that bacteria dominantly occur as biofilms in natural environments. Earlier improvements in the culture techniques are mostly done by optimizing media components. However, with technological advancement particularly in the field of genome sequencing and cell imagining techniques, new tools have become available to understand the ecophysiology of microbial communities. Hence, it becomes easier to mimic environmental conditions in the culture plate. Other methods include co-culturing, emendation of growth factors, and cultivation after physical cell sorting. Most recently, techniques have been proposed for bacterial cultivation by employing genomic data to understand either microbial interactions (network-directed targeted bacterial isolation) or ecosystem engineering (reverse genomics). Hopefully, these techniques may be applied to almost all environmental samples, and help fill the gaps between the cultured and uncultured microbial communities.
Application of in situ cultivation in marine microbial resource mining
Dawoon Jung, Liwei Liu, Shan He
2021, 3(2): 148-161.   doi: 10.1007/s42995-020-00063-x
Abstract(555) HTML PDF Springerlink
Abstract:
Microbial communities in marine habitats are regarded as underexplored reservoirs for discovering new natural products with potential application. However,only a few microbes in nature can be cultivated in the laboratory. This has led to the development of a variety of isolation and cultivation methods,and in situ cultivation is one of the most popular. Diverse in situ cultivation methods,with the same basic principle,have been applied to a variety of environmental samples. Compared with conventional approaches,these new methods are able to cultivate previously uncultured and phylogenetically novel microbes,many with biotechnological potential. This review introduces the various in situ cultivation methods for the isolation of previously uncultured microbial species and their potential for marine microbial resource mining. Furthermore,studies that investigated the key and previously unidentifed mechanisms of growing uncultivated microorganisms by in situ cultivation,which will shed light on the understanding of microbial uncultivability,were also reviewed.
FACS-iChip: a high-efficiency iChip system for microbial 'dark matter' mining
Haoze Liu, Ran Xue, Yiling Wang, Erinne Stirling, Shudi Ye, Jianming Xu, Bin Ma
2021, 3(2): 162-168.   doi: 10.1007/s42995-020-00067-7
Abstract(415) HTML PDF Springerlink
Abstract:
The isolation chip method (iChip) provides a novel approach for culturing previously uncultivable microorganisms; this method is currently limited by the user being unable to ensure single-cell loading within individual wells. To address this limitation, we integrated flow cytometry-based fluorescence-activated cell sorting with a modified iChip (FACS-iChip) to effectively mine microbial dark matter in soils. This method was used for paddy soils with the aim of mining uncultivable microorganisms and making preliminary comparisons between the cultured microorganisms and the bulk soil via 16S rRNA gene sequencing. Results showed that the FACS-iChip achieved a culture recovery rate of almost 40% and a culture retrieval rate of 25%. Although nearly 500 strains were cultured from 19 genera with 8 FACS-iChip plates, only six genera could be identified via 16S rRNA gene amplification. This result suggests that the FACS-iChip is capable of detecting strains in the currently dead spaces of PCR-based sequencing technology. We, therefore, conclude that the FACS-iChip system provides a highly efficient and readily available approach for microbial 'dark matter' mining.
One cell at a time:droplet-based microbial cultivation, screening and sequencing
Beiyu Hu, Peng Xu, Liang Ma, Dongwei Chen, Jian Wang, Xin Dai, Li Huang, Wenbin Du
2021, 3(2): 169-188.   doi: 10.1007/s42995-020-00082-8
Abstract(1809) HTML PDF Springerlink
Abstract:
Microbes thrive and, in turn, influence the earth's environment, but most are poorly understood because of our limited capacity to reveal their natural diversity and function. Developing novel tools and effective strategies are critical to ease this dilemma and will help to understand their roles in ecology and human health. Recently, droplet microfluidics is emerging as a promising technology for microbial studies with value in microbial cultivating, screening, and sequencing. This review aims to provide an overview of droplet microfluidics techniques for microbial research. First, some critical points or steps in the microfluidic system are introduced, such as droplet stabilization, manipulation, and detection. We then highlight the recent progress of droplet-based methods for microbiological applications, from high-throughput single-cell cultivation, screening to the targeted or whole-genome sequencing of single cells.
The differentiation of iron-reducing bacterial community and iron reduction activity between riverine and marine sediments in the Yellow River estuary
Hongxia Zhang, Fanghua Liu, Shiling Zheng, Lei Chen, Xiaoli Zhang, Jun Gong
2020, 2(1): 87-96.   doi: 10.1007/s42995-019-00001-6
Abstract HTML PDF(55)
Microbial assembly, interaction, functioning, activity and diversifcation: a review derived from community compositional data
Jiwen Liu, Zhe Meng, Xiaoyue Liu, Xiao-Hua Zhang
2019, 1(1): 112-128.   doi: 10.1007/s42995-019-00004-3
Abstract HTML PDF(36)
Methods for the diagnosis of bacterial fish diseases
Brian Austin
2019, 1(1): 41-49.   doi: 10.1007/s42995-019-00002-5
Abstract HTML PDF(36)
Our recent progress in epigenetic research using the model ciliate, Tetrahymena thermophila
Ting Cheng, Yuanyuan Wang, Jie Huang, Xiao Chen, Xiaolu Zhao, Shan Gao, Weibo Song
2019, 1(1): 4-14.   doi: 10.1007/s42995-019-00015-0
Abstract HTML PDF(29)
Metabolites from marine invertebrates and their symbiotic microorganisms: molecular diversity discovery, mining, and application
Lu Liu, Yao-Yao Zheng, Chang-Lun Shao, Chang-Yun Wang
2019, 1(1): 60-94.   doi: 10.1007/s42995-019-00021-2
Abstract HTML PDF(28)
Vertical variation in Vibrio community composition in Sansha Yongle Blue Hole and its ability to degrade macromolecules
Bei Li, Jiwen Liu, Shun Zhou, Liang Fu, Peng Yao, Lin Chen, Zuosheng Yang, Xiaolei Wang, Xiao-Hua Zhang
2020, 2(1): 60-72.   doi: 10.1007/s42995-019-00003-4
Abstract HTML PDF(39)
Eco-friendly preparation of chitooligosaccharides with different degrees of deacetylation from shrimp shell waste and their effects on the germination of wheat seeds
Xiaodan Fu, Lin Zhu, Li Li, Tan Zhang, Meng Li, Haijin Mou
2019, 1(1): 95-103.   doi: 10.1007/s42995-019-00012-3
Abstract HTML PDF(35)
Effects of Bacillus subtilis on hepatic lipid metabolism and oxidative stress response in grass carp (Ctenopharyngodon idellus) fed a high-fat diet
Hui Zhao, Yan'er Luo, Yongan Zhang, Xiaoxuan Chen, Hui Wang, Daoyuan Guo, Zhixin Wu
2020, 2(1): 50-59.   doi: 10.1007/s42995-019-00005-2
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Recent advances in amino acid sensing and new challenges for protein nutrition in aquaculture
Chengdong Liu, Xuan Wang, Huihui Zhou, Kangsen Mai, Gen He
2019, 1(1): 50-59.   doi: 10.1007/s42995-019-00022-1
Abstract HTML PDF(28)
Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafsh
Lei Wang, Jae Young Oh, Hye-Won Yang, Hyun Soo Kim, You-Jin Jeon
2019, 1(1): 104-111.   doi: 10.1007/s42995-019-00006-1
Abstract HTML PDF(36)

Marine Life Science & Technology

(Started in 2019)

  • Editor-in-Chief: Weibo Song
  • Executive Deputy Editor: Xiao-Hua Zhang
  • Frequency of Publication: Quarterly
  • ISSN: 2096-6490
  • eISSN: 2662-1746
  • CN:    37-1519/Q