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Eco-friendly preparation of chitooligosaccharides with different degrees of deacetylation from shrimp shell waste and their effects on the germination of wheat seeds
Xiaodan Fu, Lin Zhu, Li Li, Tan Zhang, Meng Li, Haijin Mou
2019, 1(1): 95-103.  doi: 10.1007/s42995-019-00012-3
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Recent advances in amino acid sensing and new challenges for protein nutrition in aquaculture
Chengdong Liu, Xuan Wang, Huihui Zhou, Kangsen Mai, Gen He
2019, 1(1): 50-59.  doi: 10.1007/s42995-019-00022-1
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Morphogenesis: a focus on marine invertebrates
Zhiyi Lv, Qiongxuan Lu, Bo Dong
2019, 1(1): 28-40.  doi: 10.1007/s42995-019-00016-z
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Cost-reduction strategies in massive genomics experiments
Haichao Li, Kun Wu, Chenchen Ruan, Jiao Pan, Yujin Wang, Hongan Long
2019, 1(1): 15-21.  doi: 10.1007/s42995-019-00013-2
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Our recent progress in epigenetic research using the model ciliate, Tetrahymena thermophila
Ting Cheng, Yuanyuan Wang, Jie Huang, Xiao Chen, Xiaolu Zhao, Shan Gao, Weibo Song
2019, 1(1): 4-14.  doi: 10.1007/s42995-019-00015-0
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Microbial assembly, interaction, functioning, activity and diversifcation: a review derived from community compositional data
Jiwen Liu, Zhe Meng, Xiaoyue Liu, Xiao-Hua Zhang
2019, 1(1): 112-128.  doi: 10.1007/s42995-019-00004-3
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Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafsh
Lei Wang, Jae Young Oh, Hye-Won Yang, Hyun Soo Kim, You-Jin Jeon
2019, 1(1): 104-111.  doi: 10.1007/s42995-019-00006-1
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Metabolites from marine invertebrates and their symbiotic microorganisms: molecular diversity discovery, mining, and application
Lu Liu, Yao-Yao Zheng, Chang-Lun Shao, Chang-Yun Wang
2019, 1(1): 60-94.  doi: 10.1007/s42995-019-00021-2
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Characterization of TEMPO-oxidized chitin nanofibers with various oxidation times and its application as an enzyme immobilization support
Rui Chen, Wen-Can Huang, Wei Wang, Xiangzhao Mao
Accepted Manuscript  doi: 10.1007/s42995-020-00054-y
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Abstract:
Chitin nanofibers have recently received increased attention and are considered to be a promising material for a wide range of applications because of their excellent characteristics. In this study, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized chitin nanofibers (CNFs) with various oxidation times were prepared and characterized. CNFs with different oxidation times were then utilized for enzyme immobilization, using chymotrypsin as a model enzyme. The effects of oxidation time on enzyme immobilization were explored. Results showed characteristics of chitin nanofibers can be controlled by adjusting oxidation time. CNFs treated with TEMPO for 360 min showed the lowest crystallinity (79.13 ± 1.43%), the shortest length (241.70 ± 74.61 nm), the largest width (12.67 ± 3.43 nm), and the highest transmittance (73.01% at 800 nm). The activity of immobilized enzymes and enzyme loading showed good correlation to the carboxylate content of CNFs. The enzyme eiciency based on CNFs and the content of carboxylate groups peaked at the oxidization time of 60 min. When the additional amount of chymotrypsins (CTs) was 500 or 2000 mg/g carrier, the highest loading amount of CTs was 307.17 ± 4.08 or 726.82 ± 12.05 mg/g carrier, respectively.
Progress in studies on the diversity and distribution of planktonic ciliates (Protista, Ciliophora) in the South China Sea
Weiwei Liu, Mann Kyoon Shin, Zhenzhen Yi, Yehui Tan
Corrected proof  doi: 10.1007/s42995-020-00070-y
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Abstract:
As an important component of microzooplankton, ciliates play a key role in matter cycling and energy flow in marine planktonic ecosystems. Studies of planktonic ciliate have been extensive in the South China Sea (SCS) over the last 20 years. Here, we summarize the recent progress on the diversity and distribution of this group in the SCS. This includes that in: (1) the waters covering the intertidal zone of the northern SCS, most studies have focused on taxonomy, with 71 species collected, identified, and described (with ~ 40% new species); (2) neritic waters distribution patterns have been examined at a regional scale, with ciliates displaying significant spatial variations and seasonal dynamics; (3) in oceanic waters, there has been a focus on ciliate distribution in north, centre, and south regions, where mesoscale physical processes play roles in controlling distributions, and noticeable vertical variations occur. More generally, some studies examine the influences of environment variables on ciliates, and indicate that chlorophyll a concentration is commonly positively correlated with ciliates abundance. In addition, some significant findings are summarized, the limitations of past studies are considered, and recommendations are made for future work on planktonic ciliates in SCS.
Marine urease with higher thermostability, pH and salinity tolerance from marine sponge-derived Penicillium steckii S4-4
Changrong Liu, Yao Xiao, Yilin Xiao, Zhiyong Li
Corrected proof  doi: 10.1007/s42995-020-00076-6
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Abstract:
Urease has a broad range of applications, however, the current studies on urease mainly focus on terrestrial plants or microbes. Thus, it is quite necessary to determine if marine-derived ureases have different characteristics from terrestrial origins since the finding of ureases with superior performance is of industrial interest. In this study, the marine urease produced by Penicillium steckii S4-4 derived from marine sponge Siphonochalina sp. was investigated. This marine urease exhibited a maximum specific activity of 1542.2 U mg protein-1. The molecular weight of the enzyme was 183 kDa and a single subunit of 47 kDa was detected, indicating that it was a tetramer. The N-terminal amino acid sequence of the urease was arranged as GPVLKKTKAAAV with greatest similarity to that from marine algae Ectocarpus siliculosus. This urease exhibited a Km of 7.3 mmol L-1 and a Vmax of 1.8 mmol urea min-1 mg protein-1. The optimum temperature, pH and salinity are 55 ℃, 8.5 and 10%, respectively. This urease was stable and more than 80% of its maximum specific activity was detected after incubating at 25-60 ℃ for 30 min, pH 5.5-10.0 or 0-25% salinity for 6 h. Compared with the terrestrial urease from Jack bean, this marine urease shows higher thermostability, alkaline preference and salinity tolerance, which extends the potential application fields of urease to a great extent.
Roussoelins A and B: two phenols with antioxidant capacity from ascidian-derived fungus Roussoella siamensis SYSU-MS4723
Senhua Chen, Hongjie Shen, Yanlian Deng, Heng Guo, Minghua Jiang, Zhenger Wu, Huimin Yin, Lan Liu
Corrected proof  doi: 10.1007/s42995-020-00066-8
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Abstract:
Ascidian-derived microorganisms are a significant source of pharmacologically active metabolites with interesting structural properties. When discovering bioactive molecules from ascidian-derived fungi, two new phenols, roussoelins A (1) and B (2), and ten known polyketides (312) were isolated from the ascidian-derived fungus Roussoella siamensis SYSU-MS4723. The planar structure of compounds 1 and 2 was established by analysis of HR-ESIMS and NMR data. The conformational analysis of the new compounds was assigned according to coupling constants and selective gradient NOESY experiments, and absolute configurations were completed by the modified Mosher's method. Among the isolated compounds, 1, 2, and 9 showed moderate antioxidant capacity.
Application of in situ cultivation in marine microbial resource mining
Dawoon Jung, Liwei Liu, Shan He
Corrected proof  doi: 10.1007/s42995-020-00063-x
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Abstract:
Microbial communities in marine habitats are regarded as underexplored reservoirs for discovering new natural products with potential application. However,only a few microbes in nature can be cultivated in the laboratory. This has led to the development of a variety of isolation and cultivation methods,and in situ cultivation is one of the most popular. Diverse in situ cultivation methods,with the same basic principle,have been applied to a variety of environmental samples. Compared with conventional approaches,these new methods are able to cultivate previously uncultured and phylogenetically novel microbes,many with biotechnological potential. This review introduces the various in situ cultivation methods for the isolation of previously uncultured microbial species and their potential for marine microbial resource mining. Furthermore,studies that investigated the key and previously unidentifed mechanisms of growing uncultivated microorganisms by in situ cultivation,which will shed light on the understanding of microbial uncultivability,were also reviewed.
From ecophysiology to cultivation methodology: filling the knowledge gap between uncultured and cultured microbes
Nimaichand Salam, Wen-Dong Xian, Mipeshwaree Devi Asem, Min Xiao, Wen-Jun Li
Corrected proof  doi: 10.1007/s42995-020-00064-w
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Abstract:
Earth is dominated by a myriad of microbial communities, but the majority fails to grow under in situ laboratory conditions. The basic cause of unculturability is that bacteria dominantly occur as biofilms in natural environments. Earlier improvements in the culture techniques are mostly done by optimizing media components. However, with technological advancement particularly in the field of genome sequencing and cell imagining techniques, new tools have become available to understand the ecophysiology of microbial communities. Hence, it becomes easier to mimic environmental conditions in the culture plate. Other methods include co-culturing, emendation of growth factors, and cultivation after physical cell sorting. Most recently, techniques have been proposed for bacterial cultivation by employing genomic data to understand either microbial interactions (network-directed targeted bacterial isolation) or ecosystem engineering (reverse genomics). Hopefully, these techniques may be applied to almost all environmental samples, and help fill the gaps between the cultured and uncultured microbial communities.
Report of epibenthic macrofauna found from Haima cold seeps and adjacent deep-sea habitats, South China Sea
Dong Dong, Xinzheng Li, Mei Yang, Lin Gong, Yang Li, Jixing Sui, Zhibin Gan, Qi Kou, Ning Xiao, Junlong Zhang
Corrected proof  doi: 10.1007/s42995-020-00073-9
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Abstract:
This work reports on a preliminary taxonomic study of epibenthic macroinvertebrates collected or observed by underwater video at the Haima cold seeps and in adjacent deep-sea habitats, including a mud volcano field and Ganquan Plateau, during an expedition in the South China Sea by the Chinese-manned submersible Shenhai Yongshi in May 2018. A total of 41 species belonging to 6 phyla were identified, among which 34 species were collected from the Haima cold seeps. Mollusks and crustaceans that are specialized in reducing habitats were predominant in biotopes of the Haima cold seeps, whereas sponges and cold-water corals and their commensals were prominent in communities of the mud volcano field and the slopes of Ganquan Plateau. The distribution and faunal composition of each taxonomic group are discussed.
Proteases from the marine bacteria in the genus Pseudoalteromonas: diversity, characteristics, ecological roles, and application potentials
Xiu-Lan Chen, Yan Wang, Peng Wang, Yu-Zhong Zhang
2020, 2(4): 309-323.   doi: 10.1007/s42995-020-00058-8
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Abstract:
Bacterial strains of the genus Pseudoalteromonas, which includes 48 species, are widely distributed in various ocean environments. Many strains are often abundant and the highest protease producer among marine culturable protease-secreting bacteria, suggesting their important role in marine organic nitrogen degradation and cycling. The extracellular proteases of Pseudoalteromonas are diverse, including serine proteases, metalloproteases, and cystine proteases. Pseudoalteromonas proteases have unique properties, such as cold adaptation, salt tolerance, distinct substrate specificity, and catalytic mechanism. They play important ecological roles in marine organic nitrogen degradation and in the interactions of Pseudoalteromonas with other organisms. Some Pseudoalteromonas proteases have shown promising application potentials in bioactive peptide preparation and meat processing. In this review, advances in our knowledge of Pseudoalteromonas proteases are introduced, with a focus on diversity, characters, ecological significance, and application potentials.
High abundance and reproductive output of an intertidal limpet (Siphonaria japonica) in environments with high thermal predictability
Jie Wang, Xin Peng, Yunwei Dong
2020, 2(4): 324-333.   doi: 10.1007/s42995-020-00059-7
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Abstract:
The predictability of high temperatures is important for intertidal species for coping with thermal stress. To investigate the roles of high temperature and its predictability on the population abundance and reproductive output of an intertidal rocky shore limpet, Siphonaria japonica, we monitored the operative temperature, recorded the population abundance of egg ribbons and adults, and quantified the expression of heat shock protein 70 (hsp70) mRNA on two warm-temperate rocky shores with different thermal environments. Abundances of limpets and egg ribbons in the hotter but more predictable (HP) habitats were higher than those in the relatively benign and unpredictable (BU) habitats. In the HP habitats, there was a strong positive correlation between habitat temperature and population abundance. For limpets living in the HP habitats, the expression of hsp70 exhibited a smaller increase with rising body temperature than for BU limpets, indicating the existence in HP limpets of a preparatory strategy in cellular stress response against thermal stress. Our results demonstrate that the predictability of operative temperature can affect physiological responses and population dynamics. The importance of predictability should be considered in analyses of the ecological consequences of climate warming.
Morphology, taxonomy and molecular phylogeny of three marine peritrich ciliates, including two new species: Zoothamnium apoarbuscula n. sp. and Z. apohentscheli n. sp. (Protozoa, Ciliophora, Peritrichia)
Tong Wu, Yuqing Li, Borong Lu, Zhuo Shen, Weibo Song, Alan Warren
2020, 2(4): 334-348.   doi: 10.1007/s42995-020-00046-y
Abstract(968) HTML PDF Springerlink
Abstract:
Zoothamnium is a speciose genus, most species of which have incomplete morphological data based on modern criteria. In the present study, the morphology of three species of Zoothamnium, i.e., Z. apoarbuscula n. sp., Z. apohentscheli n. sp., and Z. alternans, collected in Qingdao, China, was revealed using living observation and silver staining. In addition, the SSU rDNA of each species was sequenced for phylogenetic analyses. Zoothamnium apoarbuscula n. sp. is characterized by its umbellate colony which is up to 900 μm high, dichotomously branched stalk, differentiation of zooids, and infundibular polykinety 3 comprising three equal-length ciliary rows. Zoothamnium apohentscheli n. sp is characterized by its large colony up to 1700 μm high, alternately branched stalk, and infundibular polykinety 3 comprising three equal-length ciliary rows. A population of Z. alternans is described in detail. Phylogenetic analyses based on SSU rDNA sequence data revealed that species with an alternately branched stalk cluster together in gene trees and probably represent an independent lineage within the genus Zoothamnium.
Identification, expression analysis, and functional characterization of ghrelin and its receptors in spotted sea bass (Lateolabrax maculatus)
Peng Yu, Yangyang Zhou, Xin Qi, Hongying Fan, Kaiqiang Zhang, Xiaoyan Zhang, Yun Li, Haishen Wen
2020, 2(4): 349-359.   doi: 10.1007/s42995-020-00055-x
Abstract(179) HTML PDF Springerlink
Abstract:
Ghrelin (Ghrl), an appetite-inducing peptide hormone secreted by the stomach, is the endogenous ligand for the growth hormone secretagogue receptor (Ghs-r). In this study, we identified the preproghrelin gene and its receptors in spotted sea bass (Lateolabrax maculatus). The ghrl gene consisted of an open reading frame (ORF) of 324 nucleotides encoding 107 amino acids, and the premature protein contained a 20-amino-acid mature peptide. Through a syntenic analysis, we also validated the annotation of growth hormone secretagogue receptor 1a (ghs-r1a) and growth hormone secretagogue receptor 1a-like (ghs-r1a-like), which contained seven-transmembrane structures, in spotted sea bass. The ORF of ghs-r1a consisted of 1152 bp that encoded a 383-amino-acid protein, and ghs-r1a-like contained an ORF of 2631 bp and produced a protein with 876 amino acids. A phylogenetic analysis showed that spotted sea bass ghrl and its receptors clustered with those of other fishes and were more distantly related to those of other vertebrates. In situ hybridization revealed that ghrl was highly expressed in the stomach and localized in the mucosa and submucosa. The expression of these genes varied during short-term starvation in a time-dependent manner. In vitro studies showed that after incubation with Ghrl for 3 h enhanced the expression of motilin (mln), gastrin (gas) and cholecystokinin (cck), but this effect vanished after 6 h of incubation. In summary, Ghrl and its receptors might play important roles in the regulation of food intake in spotted sea bass.
Roles of dietary taurine in fish nutrition
W. W. H. A. Sampath, R. M. D. S. Rathnayake, Mengxi Yang, Wenbing Zhang, Kangsen Mai
2020, 2(4): 360-375.   doi: 10.1007/s42995-020-00051-1
Abstract(236) HTML PDF Springerlink
Abstract:
Taurine is a conditionally essential amino acid in fish nutrition. The present study addressed the practical application of examining published data on fish nutrition over the past 20 years, emphasizing the topic of taurine by using computational tools and their applications. According to the published articles, an increased linear growth of research occurred, with Japanese flounder being the most examined fish species. Dietary taurine supplementation has several beneficial effects in fish nutrition, such as survival, growth, feed utilization, protein and energy retention, intermediate metabolism, anti-oxidation, anti-stress, disease resistance, muscle texture and reproductive performance. Also, there are negative effects in some species. Dietary taurine exerted effects on several gene expressions and enzyme activities; these are important in taurine metabolism in fish. These genes and enzymes included taurine transporter (TauT), cysteine dioxygenase (CDO), cysteamine dioxygenase (ADO), cysteine sulfonate decarboxylase (CSD) and pretrypsinogen (Ptry). Plant protein-based diets with taurine supplementation are recommended because of the absence of taurine in plant protein.
Optimal amounts of coconut oil in diets improve the growth, antioxidant capacity and lipid metabolism of large yellow croaker (Larimichthys crocea)
Tao Ding, Ning Xu, Yongtao Liu, Xueshan Li, Xiaojun Xiang, Dan Xu, Chuanwei Yao, Qiangde Liu, Zhaoyang Yin, Kangsen Mai, Qinghui Ai
2020, 2(4): 376-385.   doi: 10.1007/s42995-020-00045-z
Abstract(301) HTML PDF Springerlink
Abstract:
The purpose of this study was to investigate the effect of different dietary coconut oil (CO) levels on growth, antioxidant capacity and lipid metabolism of juvenile large yellow croaker (Larimichthys crocea). Five iso-nitrogen (45% crude protein) and iso-lipid (13% crude lipid) experimental diets were prepared by replacing 0% (the control), 25%, 50%, 75% and 100% fish oil with coconut oil. The results showed that dietary CO had no significant effect on survival rate (SR, P > 0.05). However, the specific growth rate was increased significantly when compared with the control group when fish were fed the diet with 50% CO (P < 0.05). The saturated fatty acids were increased significantly with increasing dietary CO in the liver and muscle, whereas the content of n-3 PUFA was decreased significantly (P < 0.05). The highest activities of glutathione peroxidase and superoxide dismutase in the liver were recorded in fish-fed diet with 50% CO; conversely, the content of malondialdehyde was significantly decreased (P < 0.05). The mRNA expression of peroxisome proliferator-activated receptor α, carnitine palmitoyl transferase 1 and acyl-CoA oxidase reached the highest levels in fish-fed diet with 50% CO. To some extent, this indicated that the rapid oxidation reaction of fatty acids to provide energy may be the reason for the rapid growth of large yellow croaker. In conclusion, fish-fed diet with 50% CO increased the growth rate and antioxidant capacity. Therefore, the optimal replacement level of CO to FO in the diet should be 50%.
The differentiation of iron-reducing bacterial community and iron reduction activity between riverine and marine sediments in the Yellow River estuary
Hongxia Zhang, Fanghua Liu, Shiling Zheng, Lei Chen, Xiaoli Zhang, Jun Gong
2020, 2(1): 87-96.   doi: 10.1007/s42995-019-00001-6
Abstract HTML PDF(51)
Microbial assembly, interaction, functioning, activity and diversifcation: a review derived from community compositional data
Jiwen Liu, Zhe Meng, Xiaoyue Liu, Xiao-Hua Zhang
2019, 1(1): 112-128.   doi: 10.1007/s42995-019-00004-3
Abstract HTML PDF(28)
Methods for the diagnosis of bacterial fish diseases
Brian Austin
2019, 1(1): 41-49.   doi: 10.1007/s42995-019-00002-5
Abstract HTML PDF(34)
Our recent progress in epigenetic research using the model ciliate, Tetrahymena thermophila
Ting Cheng, Yuanyuan Wang, Jie Huang, Xiao Chen, Xiaolu Zhao, Shan Gao, Weibo Song
2019, 1(1): 4-14.   doi: 10.1007/s42995-019-00015-0
Abstract HTML PDF(20)
Metabolites from marine invertebrates and their symbiotic microorganisms: molecular diversity discovery, mining, and application
Lu Liu, Yao-Yao Zheng, Chang-Lun Shao, Chang-Yun Wang
2019, 1(1): 60-94.   doi: 10.1007/s42995-019-00021-2
Abstract HTML PDF(21)
Vertical variation in Vibrio community composition in Sansha Yongle Blue Hole and its ability to degrade macromolecules
Bei Li, Jiwen Liu, Shun Zhou, Liang Fu, Peng Yao, Lin Chen, Zuosheng Yang, Xiaolei Wang, Xiao-Hua Zhang
2020, 2(1): 60-72.   doi: 10.1007/s42995-019-00003-4
Abstract HTML PDF(34)
Eco-friendly preparation of chitooligosaccharides with different degrees of deacetylation from shrimp shell waste and their effects on the germination of wheat seeds
Xiaodan Fu, Lin Zhu, Li Li, Tan Zhang, Meng Li, Haijin Mou
2019, 1(1): 95-103.   doi: 10.1007/s42995-019-00012-3
Abstract HTML PDF(24)
Recent advances in amino acid sensing and new challenges for protein nutrition in aquaculture
Chengdong Liu, Xuan Wang, Huihui Zhou, Kangsen Mai, Gen He
2019, 1(1): 50-59.   doi: 10.1007/s42995-019-00022-1
Abstract HTML PDF(19)
Effects of Bacillus subtilis on hepatic lipid metabolism and oxidative stress response in grass carp (Ctenopharyngodon idellus) fed a high-fat diet
Hui Zhao, Yan'er Luo, Yongan Zhang, Xiaoxuan Chen, Hui Wang, Daoyuan Guo, Zhixin Wu
2020, 2(1): 50-59.   doi: 10.1007/s42995-019-00005-2
Abstract HTML PDF(18)
Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafsh
Lei Wang, Jae Young Oh, Hye-Won Yang, Hyun Soo Kim, You-Jin Jeon
2019, 1(1): 104-111.   doi: 10.1007/s42995-019-00006-1
Abstract HTML PDF(28)

Marine Life Science & Technology

(Started in 2019)

  • Editor-in-Chief: Weibo Song
  • Executive Deputy Editor: Xiao-Hua Zhang
  • Frequency of Publication: Quarterly
  • ISSN: 2096-6490
  • eISSN: 2662-1746
  • CN:    37-1519/Q